Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)

Xiaofeng Zhou, Bethany B Moore

要旨

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

材料

製品番号

ブランド

製品内容

Sigma-Aldrich

酢酸, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

塩酸, ACS reagent, 37%

Sigma-Aldrich

塩化鉄(III), reagent grade, 97%

Sigma-Aldrich

クエン酸, ACS reagent, ≥99.5%

Sigma-Aldrich

硫酸カリウムアルミニウム十二水和物, ACS reagent, ≥98%

Sigma-Aldrich

ヘマトキシリン, certified by the Biological Stain Commission

Sigma-Aldrich

エオシンY, Dye content ~99 %

Sigma-Aldrich

リン酸ナトリウム一塩基性, BioXtra, ≥99.0%