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Merck

The olfactory epithelium as a port of entry in neonatal neurolisteriosis.

Nature communications (2018-10-17)
Dennis Pägelow, Chintan Chhatbar, Andreas Beineke, Xiaokun Liu, Andreas Nerlich, Kira van Vorst, Manfred Rohde, Ulrich Kalinke, Reinhold Förster, Stephan Halle, Peter Valentin-Weigand, Mathias W Hornef, Marcus Fulde
要旨

Bacterial infections of the central nervous system (CNS) remain a major cause of mortality in the neonatal population. Commonly used parenteral infection models, however, do not reflect the early course of the disease leaving this critical step of the pathogenesis largely unexplored. Here, we analyzed nasal exposure of 1-day-old newborn mice to Listeria monocytogenes (Lm). We found that nasal, but not intragastric administration, led to early CNS infection in neonate mice. In particular, upon bacterial invasion of the olfactory epithelium, Lm subsequently spread along the sensory neurons entering the brain tissue at the cribriform plate and causing a significant influx of monocytes and neutrophils. CNS infection required listeriolysin for penetration of the olfactory epithelium and ActA, a mediator of intracellular mobility, for translocation into the brain tissue. Taken together, we propose an alternative port of entry and route of infection for neonatal neurolisteriosis and present a novel infection model to mimic the clinical features of late-onset disease in human neonates.

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製品内容

Sigma-Aldrich
D.E.R. 332, used as embedding medium
ログイン組織・契約価格を表示する
カタログ番号容量在庫状況単価数量
20 μg
1 出荷予定日 2025年2月16日
CHF 249.00
50 μg
1 出荷予定日 2025年2月16日
CHF 443.00
Sigma-Aldrich
Anti-Tubulin β III Antibody, Chemicon®, from mouse
ログイン組織・契約価格を表示する
カタログ番号容量在庫状況単価数量
20 μg
1 出荷予定日 2025年2月16日
CHF 249.00
50 μg
1 出荷予定日 2025年2月16日
CHF 443.00