コンテンツへスキップ
Merck
  • Mechanically stimulated ATP release from murine bone cells is regulated by a balance of injury and repair.

Mechanically stimulated ATP release from murine bone cells is regulated by a balance of injury and repair.

eLife (2018-10-17)
Nicholas Mikolajewicz, Elizabeth A Zimmermann, Bettina M Willie, Svetlana V Komarova
要旨

Bone cells sense and actively adapt to physical perturbations to prevent critical damage. ATP release is among the earliest cellular responses to mechanical stimulation. Mechanical stimulation of a single murine osteoblast led to the release of 70 ± 24 amole ATP, which stimulated calcium responses in neighboring cells. Osteoblasts contained ATP-rich vesicles that were released upon mechanical stimulation. Surprisingly, interventions that promoted vesicular release reduced ATP release, while inhibitors of vesicular release potentiated ATP release. Searching for an alternative ATP release route, we found that mechanical stresses induced reversible cell membrane injury in vitro and in vivo. Ca2+/PLC/PKC-dependent vesicular exocytosis facilitated membrane repair, thereby minimizing cell injury and reducing ATP release. Priming cellular repair machinery prior to mechanical stimulation reduced subsequent membrane injury and ATP release, linking cellular mechanosensitivity to prior mechanical exposure. Thus, our findings position ATP release as an integrated readout of membrane injury and repair.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
アデノシン 5′-三リン酸 マグネシウム塩, ≥95%, bacterial
Sigma-Aldrich
カルベノキソロン 二ナトリウム塩, ≥98%
Sigma-Aldrich
キナクリン 二塩酸塩, ≥90%
Sigma-Aldrich
ルシフェラーゼ Photinus pyralis(ホタル)由来, recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
Supelco
フルフェナム酸, analytical standard, for drug analysis
Sigma-Aldrich
GSK2193874, ≥98% (HPLC)
Sigma-Aldrich
ML218, ≥98% (HPLC)
Sigma-Aldrich
Bisindolylmaleimide II, ≥97% (Mixture of 2 isomers)