G1006855

UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell A160 Peptide C18 Using Ammonium Formate as a Mobile Phase Modifier

application for UHPLC

特性

• テクニック: UHPLC: suitable
• 試験パラメータ: column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U); mobile phase: [A] 75:25 (8 mM ammonium formate, pH 2.6 with formic acid:acetonitrile); [B] 50:50 (8 mM ammonium formate, pH 2.6 with formic acid:acetonitrile); (77:23, A:B); flow rate: 0.2 mL/min; column temp.: 75 °C; detector: MS, ESI(+), TIC 100-3000 m/z; injection: 0.5 μL; sample: Mixture of six insulin variants, 100 μg/mL, 8 mM ammonium formate, pH 2.6 with formic acid
• 適合性: application for UHPLC
• アプリケーション: life science and biopharma

詳細

アナリシスノート

BIOshell A160 Peptide C18 is an ideal column for reversed-phase separations of small proteins to large peptide sequences. There is over 75% sequence similarity between all six of the insulin variants in the sample; therefore, separation of these variants is achieved through the subtle differences in the polarities of surface residues on the peptides. The use of ammonium formate as a mobile phase modifier yields more efficient peaks and less signal suppression during electrospray ionization (ESI) than some traditional ion-pair reagents (trifluoroacetic acid, formic acid).

法的情報

アナライト

Insulin-Glargine
Insulin-Bovine
Insulin-Asp
Insulin-Lispro
Insulin-Human
Insulin-Porcine