G005891

HPLC Analysis of Vitamin D₂ and D₃ on Ascentis^® Express C18

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis^® Express C18, 25 cm x 4.6 mm I.D., 5 μm particles (50538-U); mobile phase: [A] acetonitrile; [B] methanol; (95:5, A:B); flow rate: 1.5 mL/min; pressure: 1726 psi (119 bar); column temp.: 35 °C; detector: UV, 265 nm; injection: 10 μL; sample: 50 μg/mL in 90:10, acetonitrile:methanol
• 適合性: application for HPLC
• アプリケーション: clinical; vitamins, nutraceuticals, and natural products

詳細

アナリシスノート

This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

Ergocalciferol, 40,000,000 USP units/g
Cholecalciferol, ≥98% (HPLC)