808641

TSKgel^® Ether-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, ether phase

• 別名: TSKgel^® Ether-5PW HPLC Column
• UNSPSCコード: 41115700
• eCl@ss: 32110501

特性

• product name: TSKgel^® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
• 物質: stainless steel
• 製品種目: TSKgel^®
• メーカー／製品名: Tosoh 08641
• パラメーター: ≤4 M salt concentration; ≤50% organic solvent; 1.2 mL/min flow rate; 10-50 °C temperature; 20 bar max. pressure
• テクニック: HPLC: suitable
• L × 内径: 7.5 cm × 7.5 mm
• マトリックス: polymer particle platform
• マトリックス活性基: ether phase
• 粒径: 10 μm
• ポアサイズ: 1000 Å
• 使用pH: 2-12
• 分離法: hydrophobic interaction (HIC)

詳細

詳細

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.TSKgel Ether-5PW, Phenyl-5PW, and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a very hydrophobic packing (e.g., phenyl in this case). We recommend an Ether-5PW column for purifying hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing rapid analyses. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

物理的形状

Shipped in water.

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法的情報

TSKgel is a registered trademark of Tosoh Corporation