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資料

原産地証明書（COO）/試験成績書（COA）

567523-U

Discovery^® HS F5 (5 µm) HPLC Columns

Name: Discovery<SUP>&#174;</SUP> HS F5 (5 µm) HPLC Columns
Brand: SUPELCO

L × I.D. 25 cm × 21.2 mm, HPLC Column

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About This Item

UNSPSCコード:

41115700

eCl@ss:

32110501

NACRES:

SB.52

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product name

Discovery^® HS F5 HPLCカラム, 5 μm particle size, L × I.D. 25 cm × 21.2 mm

物質

stainless steel column

認証

suitable for USP L43

製品種目

Discovery^®

特徴

endcapped

メーカー／製品名

Discovery^®

包装

1 ea of

標識化の程度

12% Carbon loading

パラメーター

≤70 °C temp. range
400 bar pressure (5801 psi)

テクニック

HPLC: suitable

L × 内径

25 cm × 21.2 mm

表面積

300 m²/g

表面被覆率

4 μmol/m²

不純物

<10 ppm metals

マトリックス

silica gel, high purity, spherical particle platform
fully porous particle

マトリックス活性基

PFP (pentafluorophenyl) phase

粒径

5 μm

ポアサイズ

120 Å

operating pH range

2-8

アプリケーション

food and beverages

分離法

reversed phase

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詳細

C18カラムでの方法をDiscovery® HS F5 HPLCカラムに移行するためのガイドライン：
塩基性物質は通常C18カラムよりもHS F5カラムのほうが長く保持されます。C18カラムでの分離における有機溶媒の濃度を5～10％上昇させると、通常HS F5カラムで類似した保持が再現されます。他の化合物での結果は大きく異なります。しかし、2.5未満のlog P_o/w値の溶質はC18と比べてHS F5 では通常長く保持されます。差の程度は溶質に大きく依存しています。

Discovery® HS F5の結合相は、C18カラムとは極めて異なった逆相分離を提供します。しかし、化合物は通常同じ保持時間枠内に溶出されるので、多くの場合C18カラムでの方法を容易に移行できます。

特徴および利点

独自の選択性
C18と同等の保持(強い移動相が必要な場合もあります)
優れたピ-ク形状
安定で、低ブリ-ドのLC-MS分離
粒径3~10 μmで、スケ-ルアップ可能な分離

法的情報

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.

Ilia Brondz et al.

Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)

The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein

Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.

Koji Baba et al.

Journal of chromatography. A, 1354, 109-116 (2014-06-21)

Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and

Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase.

Federica Pellati et al.

Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)

In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an

Fast high-performance liquid chromatography analysis of phenethylamine alkaloids in Citrus natural products on a pentafluorophenylpropyl stationary phase.

Federica Pellati et al.

Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)

In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts

[Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, 5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance iquid chromatography-electrochemical detection].

Jian Mao et al.

Se pu = Chinese journal of chromatography, 27(2), 216-219 (2009-07-25)

A simple method was developed for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6-OH-MTHbetaC), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) in rat brain by high performance liquid chromatography with electrochemical detection (HPLC-ECD). The separation of the sample was performed on a Discovery

資料

Discovery^® HS F5 (5 µm) HPLC Columns