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掲載情報
A. Yuryev, Humana Press, 2007, 431 pp., hard cover
詳細
本書では、PCRプライマ-設計法の基礎と特殊な手法について解説します。ゲノムスケ-ル実験、小スケ-ルでの個々のPCR増幅のいずれにも利用できる最先端の手法が掲載されています。ホ-ルゲノム研究を行っている機関、PCRを利用する装置を設計している企業、日常的にPCRを用いている個人の研究者などに役立つ1冊です。
目次
PART I: BASIC PRINCIPLES AND SOFTWARE FOR PCR PRIMER DESIGN
1. Physical Principles and visual -OMP Software for optimal PCR design
2. OLIGO 7 Primer analysis software
3. Selection for 3'-end triplets for Polymerase Chain Reaction primers
4. The Reference Point method in primer design
5. PCR primer design using statistical modeling
6. Developing a statistical model for primer design
PART II: GENOME SCALE PCR PRIMER DESIGN
7. GST-PRIME: an algorithm for genome wide primer design
8. Genome scale probe and primer design
SECTION A: REPEAT MASKING FOR PCR PRIMER DESIGNS
9. SNPbox: Web based High Throughput primer design with an eye for repetitive sequences
10. Fast masking of repeated primer binding sites in Eukaryotic genomes
SECTION B: MULTIPLEX PCR PRIMER DESIGN
11. Degenerate primer design: Theoretical analysis and the HYDEN program
12. An iterative method for selecting degenerate multilex PCR primers
13. Primer design for multiplexed genotyping
14. MultiPLEX: Automatic grouping and evaluation of PCR primers
15. Multiprimer: a system for microarry PCR primer design
SECTION C: ALLELE SPECIFIC PCR
16. Modified Oligonucleotides as tools for allele specific amplification
17. AlleleID: A pathogen detection and identification system
SECTION D: LONG PCR PRIMER DESIGN
18. Designing primers for whole genome PCR scanning using the software packagin GenoFrag: a software package for the design of primers dedicated to whole genome scanning by LR-PCR
SECTION E: DNA METHYLATION MAPPING
19. Designing PCR primer for DNA methylation mapping
20. BiSearch: ePCR tool for native or bisulfite treated genomic template
21. Graphical design of primers with PerlPrimer
Index
1. Physical Principles and visual -OMP Software for optimal PCR design
2. OLIGO 7 Primer analysis software
3. Selection for 3'-end triplets for Polymerase Chain Reaction primers
4. The Reference Point method in primer design
5. PCR primer design using statistical modeling
6. Developing a statistical model for primer design
PART II: GENOME SCALE PCR PRIMER DESIGN
7. GST-PRIME: an algorithm for genome wide primer design
8. Genome scale probe and primer design
SECTION A: REPEAT MASKING FOR PCR PRIMER DESIGNS
9. SNPbox: Web based High Throughput primer design with an eye for repetitive sequences
10. Fast masking of repeated primer binding sites in Eukaryotic genomes
SECTION B: MULTIPLEX PCR PRIMER DESIGN
11. Degenerate primer design: Theoretical analysis and the HYDEN program
12. An iterative method for selecting degenerate multilex PCR primers
13. Primer design for multiplexed genotyping
14. MultiPLEX: Automatic grouping and evaluation of PCR primers
15. Multiprimer: a system for microarry PCR primer design
SECTION C: ALLELE SPECIFIC PCR
16. Modified Oligonucleotides as tools for allele specific amplification
17. AlleleID: A pathogen detection and identification system
SECTION D: LONG PCR PRIMER DESIGN
18. Designing primers for whole genome PCR scanning using the software packagin GenoFrag: a software package for the design of primers dedicated to whole genome scanning by LR-PCR
SECTION E: DNA METHYLATION MAPPING
19. Designing PCR primer for DNA methylation mapping
20. BiSearch: ePCR tool for native or bisulfite treated genomic template
21. Graphical design of primers with PerlPrimer
Index
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