T9822

モノクロナール抗チューブリン, ポリグルタミル化 マウス宿主抗体

clone B3, purified from hybridoma cell culture

• 別名: Anti-K-ALPHA-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03792435
• Conjugate: unconjugated
• Clone: B3, monoclonal
• Application: ARR, ELISA (i), ICC, WB
• Citations: 41

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: B3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken
• technique(s): immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract
• isotype: IgM
• UniProt accession no.: P68363
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBA4A(7277), TUBB(203068) ; mouse ... Tuba1a(22142), Tubb2b(73710) ; rat ... Tuba1a(64158)

詳細

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).

Tubulin is a cellular colchicine-binding protein.

Immunogen

精製ウニLytechinus pictus精子軸糸タンパク質。

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:

• immunoprecipitation
• immunohistochemistry
• immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Physical form

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable