SAB5900201

Alpaca Anti-GFP, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• 別名: GFP, Green fluorescent protein
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: alpaca
• 品質水準: 100
• リコンビナント: expressed in E. coli
• 結合体: unconjugated
• 抗体製品の状態: affinity purified VHH antibody
• クローン: GE10, monoclonal
• アッセイ: >95% (SDS-PAGE)
• 形状: lyophilized solid
• 分子量: 13.7 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• 精製方法: immobilized metal affinity chromatography (IMAC)
• 化学種の反応性: SARS coronavirus
• 濃度: 1 mg/mL
• テクニック: ELISA: 1:2000-1:10000; immunofluorescence: 1:100-1:600; immunoprecipitation (IP): 0.5-4.0 μg; western blot: 1:1000-1:5000
• 色: colorless
• pH: ( in PBS, pH 7.4)
• アイソタイプ: VHH
• タンパク質IDアクセッション番号: BAG71039.1
• UniProtアクセッション番号: A0A059PIQ0
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.

特異性

Green fluorescence protein (GFP) and other derivatives of GFP

免疫原

Recombinant full length GFP protein

アプリケーション

ELISA, WB, IP, IF

包装

50 µg per vial / 100 µg per vial

物理的形状

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.4

再構成

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

保管および安定性

Stable for 1 year at 4°C from date of receipt.

その他情報

Storage at 4°C; Do not freeze.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable