SAB5801012

Alpaca Anti-Mouse IgG3(Fcγ) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• 別名: Recombinant Alpaca single variable domain secondary Nanobody
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: alpaca
• リコンビナント: expressed in E. coli
• 結合体: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: secondary antibodies
• クローン: recombinant monoclonal
• 形状: lyophilized solid
• 分子量: 14 kDa
• 化学種の反応性: mouse, Alpaca
• テクニック: ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• サイズ: 0.1 mL
• 色: colorless
• アイソタイプ: VHH
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Alpaca Anti-Mouse IgG3(Fcγ) single domain Antibody is designed to specifically detect mouse IgG3 Fcγ fragment. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG3(Fcγ) single domain Antibody reacts with the Fc fragment of mouse IgG3 selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

特異性

Mouse IgG3 (Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

免疫原

Recombinant Alpaca single variable domain secondary nano antibody

包装

100 μg in Glass bottle

物理的形状

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

再構成

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

保管および安定性

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

その他情報

No cross-reactivity with mouse IgG1/2a/2b, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317,H412
• 注意書き: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危険有害性の分類: Aquatic Chronic 3 - Skin Sens. 1
• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable