SAB4600373

Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF^™750 antibody produced in donkey

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: donkey
• 結合体: CF^™ 750 conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: rabbit
• 濃度: ~2 mg/mL
• テクニック: flow cytometry: 1-10 μg/mL; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1-10 μg/mL; western blot: suitable
• 蛍光検出: λ_ex 755 nm; λ_em 777 nm
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF^™750 antibody produced in donkey, is a secondary antibody developed against both heavy and light chains of rabbit immunoglobulin G (IgG). IgG is a larger molecule with a molecular weight of 150 kDa. IgG has two light chains (L) and two heavy chains (H). The light chain can be either lamba (λ) or kappa (κ) chain. Functionally, immunoglobulins have constant region and variable region. Light chain has one variable region in the N-terminal V_L and followed by one constant domain C_L. The light chain is approximately about 25 kDa. The heavy chain has one variable region in the N-terminal and followed by three constant domains. In between the first (C₁H) and second constant (C₂H) domain is a spacer hinge region, which connects both the heavy chains. Each heavy chain is about 55 kDa.

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with the near-IR fluorescent dye CF750, one of an outstanding series of CF dyes developed by Biotium. To minimize cross-reactivity, the antibody has been adsorbed against Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep serum. Our CF750-labeled antibodies are suitable for flow cytometry with 633 nm excitation. Although the absorption peak of the conjugate is at 755 nm, excitation at 633 or 635 nm still yields excellent fluorescence. In fact, our CF750 antibody conjugates are brighter and more specific than antibodies labeled with APC-Alexa Fluor 750 tandem dye. CF750 is one of our three near-IR CF dyes. It is superior to other similar near-IR dyes, such as Alexa Fluor 750, IRDye and the DyLight near-IR dye. All of our near-IR CF dyes have combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging, near-IR Western blotting and flow cytometry

特異性

Binds all rabbit IgGs (minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins)

免疫原

rabbit IgG (H+L)

生物化学的／生理学的作用

Immunoglobulin G (IgG) is a glycoproteins that binds to specific antigen with high affinity. The antigen binding site of immunoglobulins is formed by six hypervariable regions, three in the V_L domain in the light chain and three in the V_H domain in the heavy chain.

特徴および利点

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

物理的形状

PBS（0.05％ アジ化ナトリウム、50％ グリセロール、0.2％ BSA含有）

調製ノート

遮光してください。抗体溶液は使用前に穏やかに混ぜてください。

法的情報

この製品は、Biotium, Incの許可の下、シグマ アルドリッチ社が販売しています。この製品は、12/334,387、12/607,915、12/699,778、12/850,578、61/454,484を含むがこれに限定されず、Biotium, Incによって所有またはライセンス取得されている米国特許および米国特許外の対応する特許請求、または特許出願のいずれかひとつ以上によって保護されています。購入者には、その購入者によって支払われた購入価格を考慮して、購入者独自の研究のために購入量分の製品を添付の製品説明書と一致する方法で使用できるように、限定的で非独占的な譲渡不可のライセンスが与えられます。ここで明示的に認められている場合を除いて、この製品の販売により、Biotium, Incの特許権や他の知的所有権の下、暗示や禁反言によって特別に購買者にいかなるライセンスも付与または譲渡されません。購入者は、この製品を第三者に転売または譲渡してはならず、また、診断目的、治療目的、予防目的を含むがこれに限定されない商業目的では使用できません。本製品は研究専用です。診断目的などの他の目的で使用するには、Biotium, Incから個別のライセンスを取得する必要があります。この製品を研究以外の目的で使用するためのライセンス購入に関する詳細については、Biotium, Inc.にお問い合わせください。住所：3159 Corporate Place, Hayward, CA 94545、Tel： (510) 265-1027Fax： (510) 265-1352Email：btinfo@biotium.com

CF is a trademark of Biotium, Inc.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable