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抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
N52, monoclonal
分子量
~200 kDa
化学種の反応性
mouse, pig, feline, rat, monkey, bovine, human
濃度
~1.0 mg/mL
テクニック
immunoblotting: 2.5-5 μg/mL using human neuroblastoma SH-SY5Y cell line fresh lysate
immunofluorescence: suitable
immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Cerebellum sections
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
pig ... NEFH(100156492)
詳細
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the N52 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit) and is 200 kDa.
Neurofilament 200 is encoded by the gene located at human chromosome 22q12.2.
特異性
Monoclonal Anti-Neurofilament 200 (Phos. and NonPhos.) also known as Neurofilament-H or Heavy subunit, specifically recognizes an epitope on the C-terminal tail domain of Neurofilament 200, which is present on both the phosphorylated and nonphosphorylated forms of this glycoprotein. The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human , pig, mouse, rat, monkey, feline and bovine origin.
アプリケーション
Anti-Neurofilament 200 (Phos and Non-Phos) antibody has been used in
- immunoblotting
- immunofluorescence
- immunohistochemistry
生物化学的／生理学的作用
Mutation in the NEFH gene leads to the development of autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc).
Neurofilament 200 has an important function in axonal transport, axonal plasticity and maintaining the structure of neuronal cytoskeleton. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion.
物理的形状
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
保管および安定性
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4200705-VAR:
SAB4200705-BULK:
SAB4200705-100UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Natalia Dubrowinskaja et al.
Cancer medicine, 3(2), 300-309 (2014-01-28)
Neurofilament Heavy polypeptid (NEFH) belongs to the group of type IV intermediate filament proteins. DNA methylation of the NEFH promoter and loss of expression have previously been shown to activate the AKT/β-catenin pathway in tumor cells. When identifying hypermethylation of
Kenji Kashiwagi et al.
Investigative ophthalmology & visual science, 44(1), 154-159 (2002-12-31)
To investigate the phosphorylation of the heavy neurofilament subunit (NF-H), which could be deeply involved in axonal transport of retinal ganglion cells (RGCs), in an experimental glaucoma model of chronic elevation of intraocular pressure (IOP) in monkeys. One eye in
Dissociation of axonal neurofilament content from its transport rate
Yuan A, et al.
Testing, 10(7), e0133848-e0133848 (2015)
Alterations of a 200 kDa neurofilament in the rat hippocampus after forebrain ischemia
Kaku Y, et al.
Journal of Cerebral Blood Flow and Metabolism, 13(3), 402-408 (1993)
Jia-Hui Sun et al.
Neural regeneration research, 17(5), 1023-1033 (2021-09-25)
The formation of nerve bundles, which is partially regulated by neural cell adhesion molecule 1 (NCAM1), is important for neural network organization during peripheral nerve regeneration. However, little is known about how the extracellular matrix (ECM) microenvironment affects this process.
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