SAB4200597

Monoclonal Anti-FMR1 antibody produced in mouse

clone FMR2.5, hybridoma cell culture supernatant

• 別名: FMRP, FRAXA, POF, POF1, fragile X mental retardation 1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: FMR2.5, monoclonal
• 形状: buffered aqueous solution
• 分子量: ~71 kDa
• 化学種の反応性: rat, human, mouse, hamster
• 濃度: ~1 mg/mL
• テクニック: flow cytometry: 2.0-5.0 μg/test using HeLa cells; immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts.; immunocytochemistry: suitable; immunofluorescence: 5-10 μg/mL using HepG2 cells.
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q06787
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FMR1(2332) ; mouse ... Fmr1(14265) ; rat ... Fmr1(24948)

詳細

詳細

Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.

特異性

Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1

アプリケーション

Monoclonal Anti-FMR1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

生物化学的／生理学的作用

Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable