コンテンツへスキップ
Merck

R6878

Sigma-Aldrich

モノクロナール抗リン酸化-網膜芽細胞腫 (pSer795) マウス宿主抗体

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

別名:

抗Rb, リン酸化型(pSer795)抗体

ログイン組織・契約価格を表示する


About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

RB-10, monoclonal

形状

buffered aqueous solution

分子量

antigen 110 kDa

化学種の反応性

mouse, rat, human, bovine, hamster

濃度

~2 mg/mL

テクニック

immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361

アイソタイプ

IgG1

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

phosphorylation (pSer795)

遺伝子情報

human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)

詳細

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

免疫原

ヒト網膜芽細胞腫タンパク質のC末端アミノ酸791~804(pSer795)に相当する合成リン酸化ペプチドのKLH結合体。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunofluorescence
  • fluorescence-activated cell sorting (FACS) assays
  • enzyme linked immunosorbent assay (ELISA)

生物化学的/生理学的作用

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS795 is required to inactivate Rb imposed growth suppression.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

10 - Combustible liquids


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

R6878-VAR:
R6878-BULK:
R6878-.1ML:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

The E2F1-3 transcription factors are essential for cellular proliferation
Wu L, et al.
Nature, 414(6862), 457-457 (2001)
Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells
Ohmine S, et al.
Oncology Reports, 39(4), 1725-1730 (2018)
Karen J Meaburn et al.
Aging cell, 6(2), 139-153 (2007-02-06)
A number of diseases associated with specific tissue degeneration and premature aging have mutations in the nuclear envelope proteins A-type lamins or emerin. Those diseases with A-type lamin mutation are inclusively termed laminopathies. Due to various hypothetical roles of nuclear
Cellular mechanisms of tumour suppression by the retinoblastoma gene
Burkhart DL and Sage J
Nature Reviews. Cancer, 8(9), 671-671 (2008)
Yan Li et al.
Molecular medicine reports, 14(3), 2222-2230 (2016-07-20)
Ginsenoside Rg3 (Rg3) is one of the primary constituents isolated from ginseng, and has been found to exhibit cytotoxic effects against cancer cells. The present study aimed to investigate the effects of Rg3 on human multiple myeloma cell proliferation and

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)