P6874

Anti-p53 antibody, Mouse monoclonal

clone DO-1, purified from hybridoma cell culture

• 別名: Anti-BCC7, Anti-BMFS5, Anti-LFS1, Anti-P53, Anti-TRP53
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: DO-1, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~53 kDa
• 化学種の反応性: human
• 濃度: ~2 mg/mL
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P04637
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TP53(7157)

詳細

詳細

Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.

特異性

Mouse anti-(p53) antibody reacts specifically with (p53) if human.

免疫原

recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

アプリケーション

Anti-p53 antibody, Mouse monoclonal has been used in:

• semi-dry western blotting
• immunoblotting
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunohistochemistry
• immunoprecipitation
• flow cytometry
• chromatin immunoprecipitation (ChIP) assay

生物化学的／生理学的作用

The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21^awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)