P6493

抗PKR, リン酸化型(pThr⁴⁵¹)抗体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: 抗インターフェロン誘導性二本鎖RNA依存性タンパク質, リン酸化型(pThr⁴⁵¹)抗体
• MDL番号: MFCD03455798
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous glycerol solution
• 分子量: antigen 65-68 kDa
• 化学種の反応性: mouse, human
• テクニック: immunoblotting: suitable using human HeLa cells stimulated with IFNγ and calyculin
• UniProtアクセッション番号: P19525
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pThr451)
• 遺伝子情報: human ... EIF2AK2(5610) ; mouse ... Eif2ak2(19106)

詳細

詳細

Protein kinase regulated by RNA (PKR) is an interferon-inducible ser/thr protein kinase, activated by stress signals and double-stranded RNA (dsRNA). In response to viral infection, PKR is activated by dsRNA-mediated dimerization and autophosphorylation. α-subunit of protein synthesis initiation factor 2 (eIF-2α) is well-studied substrate of PKR. Activation by PKR leads to phosphorylation on Ser51 that result in inhibition of translation. Human PKR contains at least 15 autophosphorylation sites. But phosphorylation on threonine 446 and 451 in the PKR activation loop is required ^{in vivo} and in vitro for high-level kinase activity. PKR is implicated in signalling pathways that involve NF-κB, p38, and IRF-3 and is the key effector in IFN-induced anti-viral responses, apoptosis and protein synthesis.
Anti-Phospho-PKR [pThr⁴⁵¹] specifically recognizes PKR phosphorylated on threonine 451 (65-68 kDa). The antibody detects human and mouse PKR [pThr⁴⁵¹].

免疫原

PKRのスレオニン451がリン酸化された領域に由来する合成リン酸化ペプチド。

アプリケーション

Anti-phospho-PKR (pThr⁴⁵¹) antibody may be used for detection by immunoblotting at a working dilution of 1:1,000 using human HeLa cells stimulated with IFN·γ and calyculin. For detection by immunoblotting in human HeLa cells, a working concentration of 0.1-1.0 μg/mL is recommended. Detection of phospho-PKR (pThr⁴⁵¹) was done in peritoneal macrophages obtained from thioglycolate-treated mice and human peripheral blood mononuclear cells at a working dilution of 1:500

物理的形状

D-PBS溶液 (Mg²⁺およびCa²⁺不含, pH 7.3(+/-0.1), 50%グリセロ-ル, 1.0 mg/mL BSA(IgG, プロテア-ゼフリ-)キャリア用, 0.05%アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable