P5810

モノクローナル抗CD3抗体-PE標識 マウス宿主抗体

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

• 別名: モノクロナール抗CD3
• UNSPSCコード: 12352203

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: UCHT-1, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human
• テクニック: flow cytometry: 10 μL using 1 × 10⁶ cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P07766
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD3E(916)

詳細

詳細

Mouse monoclonal clone UCHT-1 PE conjugated anti-Human CD3 recognizes the CD3 complex which is composed of 5 chains designated g, d, e, z and h having a molecular mass distribution of 16, 20, and 25 - 28 kDa. The antibody stains the cytoplasm of cerebellar Purkinje cells but does not stain B lymphocytes, monocytes, granulocytes, or NK cells. The epitope recognized by clone UCHT-1 is expressed on the e-chain of the CD3 antigen/T cell receptor complex. Detection of the epitope appears to be dependent of the binding to CD3-g or CD3-s. The epitope is sensitive to routine formalin fixation and paraffin embedding. Cryostat sections post-fixed in formalin can also be stained.

The CD3 human lymphocyte surface antigen is a glycoprotein thought to be associated with the T cell antigen receptor and to be involved in transmission of activation signals. The CD3 antigen is present on 60-80% of normal peripheral blood mononuclear cells, 20-40% of normal spleen cells, 40% of normal thymocytes, the majority of T-CLL and approximately 70% of T-ALL.

特異性

CD3複合体を認識します。クロ-ン番号UCHT-1のモノクロ-ナル抗体によって認識されるエピト-プは、CD3抗原/T細胞レセプタ-複合体のε鎖上に発現しています。3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

免疫原

ヒト胸腺細胞、セザリ-T細胞。

アプリケーション

Mouse monoclonal clone UCHT-1 PE conjugated anti-Human CD3 may be used for: enumeration of total T lymphocytes in bone marrow, blood, and other body fluids; identification and localization of normal and malignant T lymphocytes, lymphoid, and other tissues; identification of leukemias and lymphomas of T cell origin and T lymphocyte activation studies

ターゲットの説明

CD3複合体(16/20/29/22/25~28 kDa)は、たがいに結合した5本のポリペプチド鎖からなり、T細胞レセプタ-と結合します。

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

調製ノート

R-フィコエリスリン(PE)とのコンジュゲ-ションにより作製しています。この橙赤色の色素は、励起波長488 nm, 蛍光波長578 nmによる測定が効果的です。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)