MAK197

Succinate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

• 別名: SDH Activity Assay Kit
• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 検出方法: colorimetric
• 関連疾患: neurological disorders
• 保管温度: −20°C
• 遺伝子情報: human ... SDHA(6389) , SDHAF1(644096) , SDHAF2(54949) , SDHB(6390) , SDHC(6391) , SDHD(6392) ; mouse ... SDHA(66945) , SDHAF1(68332) , SDHAF2(66072) , SDHB(67680) , SDHC(66052) , SDHD(66925) ; rat ... SDHA(157074) , SDHAF2(361726) , SDHB(298596) , SDHC(289217) , SDHD(363061)

詳細

詳細

Succinate dehydrogenase (SDH; EC 1.3.5.1) is a mitochondrial enzyme that catalyzes the oxidation of succinate to fumarate and carries electrons from FADH to CoQ in eukaryotes and bacteria. It has a central function in the maintenance of cellular energy metabolism via the Krebs (tricarboxylic acid) cycle and the electron transport chain. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome.

アプリケーション

Succinate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of succinate dehydrogenase in samples.

適合性

Suitable for the detection of Succinate Dehydrogenase Activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

原理

The Succinate Dehydrogenase Activity Colorimetric Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

キットの構成要素のみ

• SDH Assay Buffer
• SDH Substrate Mix
• SDH Probe
• DCIP Standard, 2 mM
• SDH Positive Control

安全性情報

• 保管分類コード: 10 - Combustible liquids