The methylcellulose products available are not currently tested for use in spheroid culture. However, a literature search found that item M0512 has been used for culturing spheroids. Here are references for the preparation of methylcellulose in media:
"Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique" by Matthew J. Ware, PhD, et al.
"Methods in Endothelial Cell Biology," pg 56 by Prof. Dr. Hellmut G. Augustin, Department of Vascular Biology & Angiogenesis Research, Tumor Biology Center at the University of Freiburg.
"Rapid and Stable Formation Method of Human Astrocyte Spheroid in a High Viscous Methylcellulose Medium and Its Functional Advantages" by Fumiya Tao et al.
M7027
メチルセルロース
viscosity: 15 cP, BioReagent, suitable for cell culture
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
100 g | 出荷予測2026年6月01日from市川市大町368 | ￥21,500 |
250 g | カートの在庫状況を確認する | ￥31,600 |
この商品について
biological source
synthetic (organic)
Quality Segment
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
viscosity
12-18 cP, 2 % in H2O(20 °C)
solubility
water: 50 mg/mL, clear to hazy
SMILES string
[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]
InChI key
YLGXILFCIXHCMC-JHGZEJCSSA-N
General description
メチルセルロースは、セルロースのエーテル化により得られます。この市販セルロースエーテルは、両親媒性と元来の物理化学的性質を示します。メチルセルロースは、酵素耐性があり、非消化性、非アレルギー性、無毒です。
Application
メチルセルロースは、CFU-G、CFU-GM、細胞などのコロニー形成細胞ならびに毒性アッセイに使用する他の細胞の培養に用いる,細胞培養ゲルのような半固体物質の作製に用いられます。低粘度で、プラーク形成アッセイやクローニングには用いられません。
メチルセルロースは、次の用途で使用されています：
- 経口経管栄養におけるメトホルミン剤の担体として
- 腫瘍様塊培養のための乳腺上皮細胞増殖培地の成分として
- ハンギングドロップ法を用いるスフェロイド侵襲アッセイのためのスフェロイド3D共培養におけるサプリメントとして
Biochem/physiol Actions
メチルセルロースは、乳化材、食感改良剤、増粘剤、ゲル化剤などとして使用される食品産業をはじめとするさまざまな業界で使用されています。メチルセルロースは、特徴的な高粘度を得るためにシャンプー、スタイリング製品、液体せっけん、ボディーソープ、ローション、クリーム、歯磨き粉などさまざまな化粧品やパーソナルケア製品にも使用されます。さらに、医薬品の添加剤として機能し、栄養補助食品のカプセルの製造に使用されています。メチルセルロースは、凍結保存培地でグリセロールの代替品として使用されます。複数または単数の系統に関連する造血前駆細胞における増殖と分化を強化するために、培地サプリメントと併用されます。このセルロース誘導体は、懸濁液中で培養されたヒト皮膚線維芽細胞、ヒト黒色腫細胞、マウス胚線維芽細胞などのさまざまな細胞型の接着性や細胞機能の喪失を防ぐ可能性もあります。
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当該品目 | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source synthetic (organic) | biological source - | biological source - | biological source wood (pulp cellulose) |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| solubility water: 50 mg/mL, clear to hazy | solubility - | solubility - | solubility water: 20 mg/mL, clear to hazy, colorless to light yellow |
| form powder | form powder | form powder | form powder |
| product line BioReagent | product line - | product line - | product line - |
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保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which product is suitable for spheroid culture and How can I prepare DMEM media with 0,24% (w/v) for spheroid culture?
1 回答-
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How to practically reach a 1% solution of Methylcellulose in cell culture media that can't be heated excessively?
1 回答-
This product is supplied in powder form. The solubility in water is 50 mg/mL, resulting in a hazy solution. To prepare a 1% Methylcellulose solution in cell culture media from this stock solution, follow these steps:
A 1% solution requires 1 g of solute per 100 mL of solution, equivalent to 10 mg/mL. The stock solution is 50 mg/mL. To achieve this, take 20 mL of the 50 mg/mL stock solution and dilute it to a total volume of 100 mL.役に立ちましたか？
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I would like to use methylcellulose in my cell culture media to optimize organoids in suspension. What methylcellulose product would be the most appropriate? And can I dissolve it directly into the cold media?
1 回答-
The methylcellulose products available are not currently tested for use in organoid suspension. However, a previously available product, S3812, was an IMDM-based stem cell culture medium that included a methylcellulose component with a 4000 cps viscosity, at a concentration of 1%. This would correspond to product M0512. The solubility in cold medium has not been determined. Please see the link below to review the general methylcellulose datasheet which includes a viscosity comparison table:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/183/010/m6385pis.pdf役に立ちましたか？
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Can the M7027 pass through a 0.2um filter?
1 回答-
Methyl cellulose has a high molecular weight, and the viscosity of methyl cellulose solutions can also impede its ability to pass through such fine filters, as it can clog the filter pores. For sterilization, a 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of solution.
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Does this product need to be sterilized after dissolving for use in cell culture?
1 回答-
Solutions of this material require sterilization for cell culture applications. A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of the solution.
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Hello sir, I need to know molar mass or molecular weight of this code of Methyl cellulose or tell me how can i calculate can you help me?
1 回答-
The molecular weight is not analyzed on a lot to lot basis. The approximate molecular weight is 88,000 Da with methoxy substitution between 27.5 - 31.5% (weight).
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Can I use this to make 0.5% solution in water which will be used as the solvent to dissolve neratinib and administrate to mice through oral gavage?
1 回答-
This product can be solubilized in water at 50mg/ml which is a 0.5% solution. This product is tested for use in cell cultue, however it has not been tested for in vivo applications. Suitability for in vivo use will need to be validated by the end-user. See the link below for a general product information sheet for Methyl Cellulose. Note product M7140, which is the general research grade of this product. All details for M7140 will apply to M7027.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/785/m0512pis.pdf役に立ちましたか？
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How can I make a solution of Methyl cellulose, Product M7027?
1 回答-
There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols.
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Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?
1 回答-
Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media. Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.
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How do I store a stock solution of Methyl cellulose, Product M7027?
1 回答-
A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.
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