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Merck

GE11-0034-94

HiTrap® MabSelect SuRe

Cytiva 11-0034-94, pack of 1 × 5 mL

別名:

HiTrap MabSelect SuRe, MabSelect SuRe Column

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

alkali-stabilized protein A-derived (E. coli)

包装

pack of 1 × 5 mL

メーカー/製品名

Cytiva 11-0034-94

保管条件

(20% Ehtanol)
(20% Ethanol)

パラメーター

<20 mL/min flow rate
42 psi

ベッドサイズ

16 mm × 25 mm

ベッド容積

5 mL

カラム内径

16 mm

Matrix

highly cross-linked agarose

平均部品サイズ

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-13.7

動作範囲

3-12

キャパシティ

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

適合性

suitable for bioprocess medium

保管温度

2-8°C

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詳細

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).

HiTrap® MabSelect columns are prepacked with MabSelect a bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva
SuRe is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

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