G6532

抗GroEL ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗GroEL
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: E. coli
• テクニック: indirect ELISA: 1:40,000-1:80,000; western blot: 1:80,000
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Escherichia coli ... groEL(913705) , groEL(913705) ; Escherichia coli K12 ... Grol(948665)

詳細

詳細

GroEL protein belongs to the folding chaperonin family. It is a 58.3 kDa protein with apical, intermediate and equatorial functional domains.

特異性

The antibody does not cross-react with GroES and bovine HSP 70.

免疫原

purified recombinant GroEL produced in E. coli.

アプリケーション

Anti-GroEL antibody produced in rabbit has been used as positive control in histology and immunohistochemistry of Dirofilaria immitis, nematode worms and in the western blot analysis of the HeLa cells.

生物化学的／生理学的作用

GroEL, together with GroES, in the presence of ATP, assists in protein folding. Co-expression of aldehyde dehydrogenase with GroEL/GroES promotes folding and higher production of protein in soluble form. GroES and GroEL bind to the protein substrate, enclosing it in a cage like fold. The target protein after folding gets dissociated from the cage and is accompanied with hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP).

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)