F4424

モノクローナル抗Fas (CD95/Apo-1)抗体 マウス宿主抗体

clone DX2, purified from hybridoma cell culture

• 別名: モノクローナル抗Fas抗体, 抗Apo-1抗体, 抗CD95抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: DX2, monoclonal
• 詳細: fluorescence intensity and maximum percentage positive similar to that obtained with saturating antibody levels
• 形状: buffered aqueous solution
• 使用法: 10 μL sufficient for 1 × 10⁶ cells
• 化学種の反応性: human
• テクニック: flow cytometry: 4-20 μg/mL using cultured human Burkitt’s lymphoma Raji cells; microarray: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P25445
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FAS(355)

詳細

詳細

Monoclonal Anti-Human Fas (CD95/Apo-1) (mouse IgG1 isotype) is derived from the DX2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from C3H mice immunized with murine L cells transfected by a human Fas/CD95 cDNA. CD95/Fas/Apo-1 exhibits strong homologies with the extracellular domain of receptors belonging to the tumor necrosis factor (TNF) receptor family, which includes TNF receptor types 1 and 2 (TNFR1/2), the low affinity nerve growth factor receptor, and lymphocyte receptors such as CD27, CD30, CD40, and OX40. Fas is an integral membrane protein, with strong homology to TNF-α and -β , has been identified as Fas ligand.

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these results in activation of the caspases. However, Fas-mediated death occurs much more rapidly than that triggered by TNFR1. Human Fas/CD95/Apo-1 is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa).

特異性

ヒトFas(CD95/Apo-1)抗原の機能性のエピト-プと特異的に反応します。イムノブロッティングにおいて、このモノクロ-ナル抗体は変性、非還元状態のリコンビナントヒトFas(アミノ酸残基1-173)を認識します。抗体はフロ-サイトメトリ-においても反応性があるほか、アポト-シスの誘導に作用するとみられています。

免疫原

murine L cells transfected with a human Fas/CD95 cDNA.

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Fas (CD95/Apo-1) antibody is suitable for apoptosis treatment of Jurkat cells to study the contribution of channel type into the net K⁺ flux. It is also suitable for flow cytometry at a concentration of 4-20μg/mL using cultured human Burkitt′s lymphoma Raji cells.

Monoclonal Anti-Fas (CD95/Apo-1) antibody produced in mouse has been used in:

• immunoblotting
• flow cytometry
• the induction of apoptosis

生物化学的／生理学的作用

Fas is expressed in a number of lymphoma cell lines, on Epstein-Barr virus-transformed B lymphoblasts and on a proportion of activated B and T cells. Fas is also detected in soluble form and this form of the protein is thought to play a role in regulating certain aspects of immune system function. Elevated levels of soluble Fas is observed in leukemia and systemic lupus erythematosus. Therefore, altered levels of secreted Fas protein is likely to be involved in the abnormal growth regulation of lymphoid cells.

Human CD95/Fas/Apo-1 antigen is a single transmembrane glycoprotein receptor of 325 amino acids (45-48 kDa) which activate cell apoptosis. The action of Fas is mediated via FADD (Fas-associated death domain)/ MORT1, an adapter protein that has a death domain at its C-terminus and binds to the cytoplasmic death domain of Fas. APO-1/Fas(CD95) comprises of a death domain (DD) within the cytoplasmic region which triggers apoptosis upon binding of their cognate ligands. Once it is activated, APO-1/Fas(CD95) further aggregates its intracellular death domains which leads to the recruitment of two key signaling proteins followed by the formation of death-inducing signaling complex. These complex crosslinks through its C-terminal DD with APO-1/Fas receptors and engage caspase-8 via its N-terminal death effector domain (DED) to the DISC.

物理的形状

バイオリアクタ-培養上清の0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)