F2883

抗マウスIgG (全分子) F(ab′)₂ フラグメント–FITC ヒツジ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Anti-Mouse IgG FITC
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162642
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF (d)
• Citations: 31

特性

• biological source: sheep
• Quality Segment: 300
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct immunofluorescence: 1:128
• storage temp.: 2-8°C
• target post-translational modification: unmodified

詳細

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment-FITC antibody is specific for ) F(ab′)₂ fragment of mouse IgG1, G2a, G2b, and G3 subclasses. The antibody preparation is solid phase adsorbed with human serum proteins prior to conjugation to minimize cross reactivity. The antibody preparation is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.

Immunogen

Mouse IgG

Pepsin digest of antiserum

Application

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment-FITC antibody may be used in direct immunofluorescence at a minimum antibody dilution of 1:128. The antibody was used at a working dilution of 1:25 to stain microtubules in epidermal cells of Vigna unguiculata or cowpea It was also used for flow cytometry analysis using COS cells and human peripheral blood mononuclear cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Biochem/physiol Actions

全てのマウスIg分子に結合します。
Fcレセプタ-の存在によるバックグラウンド染色を回避する場合に有用です。

Physical form

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

Preparation Note

バックグラウンド染色を低減するため、ヒトサンプルで吸収済みです。

Other Notes

抗体はヒト血清タンパクで吸収済みです。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 12 - Non Combustible Liquids
• 一体何が起きているの？: nwg