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Merck

A3563

Sigma-Aldrich

抗マウスIgG (全分子) F(ab′)2フラグメント-アルカリフォスファターゼ ヒツジ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

由来生物

sheep

結合体

alkaline phosphatase conjugate

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

secondary antibodies

クローン

polyclonal

形状

buffered aqueous glycerol solution

化学種の反応性

mouse

テクニック

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

輸送温度

wet ice

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

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詳細

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Sheep anti-mouse IgG (whole molecule) F(ab′)2 fragment is specific for the F(ab′)2 of mouse IgG. The antibody preparation consists only of the F(ab′)2 fragment of sheep IgG as determined by SDS-PAGE. No contamination with sheep IgG whole molecule is observed.

免疫原

Mouse IgG

アプリケーション

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000), western blot (1:30,000), and immunohistochemistry (at 1:50 dilution in formalin-fixed, paraffin-embedded sections). The antibody may also be used for dot blot and immunoblot assays using a working dilution of 1:30,000.
ELISAs using alkaline phosphatase conjugated sheep anti-mouse IgG whole molecule antibody were performed to measure the antibody response in the sera of mice immunixed to T. crassiceps. 1:1000 at 37 degrees for one hour and developed using p-nitrophenyl (Sigma).

物理的形状

0.05M Tris溶液 (pH 8.0, 1%BSA, 1mM MgCl2, 50%グリセロ-ル, 0.1%アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

A3563-1ML:
A3563-BULK:
A3563-.5ML:
A3563PROC:
A3563-VAR:
A3563-.25ML:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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文書ライブラリにアクセスする

Rajeev K Tyagi et al.
Scientific reports, 7, 41083-41083 (2017-02-16)
Targeting of myeloid-dendritic cell receptor DC-SIGN by numerous chronic infectious agents, including Porphyromonas gingivalis, is shown to drive-differentiation of monocytes into dysfunctional mDCs. These mDCs exhibit alterations of their fine-tuned homeostatic function and contribute to dysregulated immune-responses. Here, we utilize
Pachiappan Arjunan et al.
Scientific reports, 8(1), 16607-16607 (2018-11-11)
Chronic periodontitis (CP) is a microbial dysbiotic disease linked to increased risk of oral squamous cell carcinomas (OSCCs). To address the underlying mechanisms, mouse and human cell infection models and human biopsy samples were employed. We show that the 'keystone'
Raymond Schuch et al.
PloS one, 8(4), e60754-e60754 (2013-04-18)
We identified an essential cell wall biosynthetic enzyme in Bacillus anthracis and an inhibitor thereof to which the organism did not spontaneously evolve measurable resistance. This work is based on the exquisite binding specificity of bacteriophage-encoded cell wall-hydrolytic lysins, which
Heather J Imrie et al.
PloS one, 7(11), e49816-e49816 (2012-11-28)
Haptoglobin related protein (Hpr) is a key component of trypanosome lytic factors (TLF), a subset of high-density lipoproteins (HDL) that form the first line of human defence against African trypanosomes. Hpr, like haptoglobin (Hp) can bind to hemoglobin (Hb) and
Ittai Fattal et al.
Immunology, 141(2), 276-285 (2013-10-30)
Systemic lupus erythematosus (SLE) is an autoimmune disease that can attack many different body organs; the triggering event is unknown. SLE has been associated with more than 100 different autoantibody reactivities - anti-dsDNA is prominent. Nevertheless, autoantibodies to dsDNA occur

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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