Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
EC700
GenElute™-E Organic Solvent DNA Cleanup Kit
Reagents and consumables for organic solvent clean-up from DNA. 10, 50 or 250 purifications.
別名:
GenElute™-E DNA Cleanup Kit, GenElute™-E kits
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この商品について
NACRES:
NA.52
UNSPSC Code:
41105600
Technique(s):
DNA purification: suitable
Storage temp.:
room temp
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purified by
(Single-spin negative chromotography), (Time: 3 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the removal of organic solvent traces from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.)
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
input
RNA
test parameters
: 3 min hands on time, sample volume: 90-110 μL
greener alternative category
, Aligned
storage temp.
room temp
General description
DNA/RNA preparation by traditional methods frequently contains residual phenol and/or chloroform. The GenElute™-E Organic Solvent DNA Cleanup Kit is a one-step cleanup kit for the depletion of organic solvent traces, including phenol, TRIzol reagent, chloroform, and ethanol, from DNA solutions. Ideally suited for a reliable clean-up when a universal preparation for NGS library prep is desired or required.
GenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.
With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as PCR.
Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 μl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.
GenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.
With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as PCR.
Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 μl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
The GenElute™-E Organic Solvent DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.
Features and Benefits
Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Preparation Note
Use within 12 months of receipt.
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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当該品目 | |||
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) RNA purification: suitable | technique(s) DNA purification: suitable |
| input RNA | input - | input DNA | input cells |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. 2-8°C |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category , Aligned | greener alternative category | greener alternative category | greener alternative category |
| purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 30 minutes or less) |
キットの構成要素のみ
製品番号
詳細
- Organic Solveent DNA Cleanup Spin Columns
- 1x Tris Buffer
保管分類
10 - Combustible liquids
wgk
WGK 3
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 回答-
役に立ちましたか？
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 回答-
This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
役に立ちましたか？
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 回答-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
役に立ちましたか？
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Does the technology introduce any bias into the sample?
1 回答-
GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
役に立ちましたか？
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