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Merck

EC600

Sigma-Aldrich

GenElute-E Single Spin DNA Cleanup Kit

greener alternative

別名:

E Single Spin DNA Cleanup Kit, GenElute DNA Cleanup Kit

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About This Item

UNSPSCコード:
41105600
NACRES:
NA.52

精製方法

(Single-spin negative chromotography)
(Time: 3 minutes or less)

特徴

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)

環境により配慮した代替製品の特徴

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

テクニック

DNA purification: suitable

環境により配慮した代替製品カテゴリ

保管温度

room temp

詳細

The GenElute-E Single Spin DNA Cleanup Kit is a one-step cleanup kit for the depletion of impurities and partial fractions (<50 bp) from pre-purified samples of DNA. The kit efficiently removes salt, detergent, nucleotides, primers, proteins/enzymes, organic solvents and many other inhibitors of downstream applications. The standard protocol, which can be completed in 3 minutes or less, is suitable for DNA fragments, plasmid DNA, and genomic DNA.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

アプリケーション

Traditional methods for purifying genomic DNA rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute-E Single Spin DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.

特徴および利点

Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

保管および安定性

Use within 12 months of receipt.

その他情報

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised.  GenElute-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.

法的情報

GenElute is a trademark of Sigma-Aldrich Co. LLC

保管分類コード

10 - Combustible liquids

WGK

WGK 3


試験成績書(COA)

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資料

DNA定量の精度・下流酵素プロセスに対する精製法の影響GenElute™-E DNA精製キットを用いた、UV分光測定、ゲル電気泳動、および下流のqPCRによるゲノムDNAの純度の評価

GenElute™-E DNA purification kits ensure accurate DNA quantitation and downstream enzymatic processes.

関連コンテンツ

GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.

大きな技術的進歩により、モノクローナル抗体のより迅速で効率的な製造が実現しました。抗体発見のために確立されたプラットフォームは3つあります。メルクは、それぞれの方法を使ったモノクローナル抗体ライブラリー製造のための試薬をご提供しています。

Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.

Animation showing technology principle of GenElute™-E single spin negative chromatography nucleic acid purification kits

すべて表示

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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