GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
EC600
GenElute™-E Single Spin DNA Cleanup Kit
Reagents and consumables for clean-up of pre-purified DNA. 10, 50 or 250 purifications.
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別名:
E Single Spin DNA Cleanup Kit, GenElute™ DNA Cleanup Kit
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この商品について
NACRES:
NA.52
UNSPSC Code:
41105600
現在、価格および在庫状況を閲覧できません。
purified by
(Single-spin negative chromotography), (Time: 3 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.)
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
: 3 min hands on time, sample volume: 90-110 μL
greener alternative category
storage temp.
room temp
General description
The GenElute™-E Single Spin DNA Cleanup Kit is a one-step cleanup kit for the depletion of impurities and partial fractions (<50 bp) from pre-purified samples of DNA. The kit efficiently removes salt, detergent, nucleotides, primers, proteins/enzymes, organic solvents and many other inhibitors of downstream applications. The standard protocol, which can be completed in 3 minutes or less, is suitable for DNA fragments, plasmid DNA, and genomic DNA.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
Traditional methods for purifying genomic DNA rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute™-E Single Spin DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.
Features and Benefits
Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Preparation Note
Use within 12 months of receipt.
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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当該品目 | ||
|---|---|---|
| description Reagents and consumables for clean-up of pre-purified DNA. 10, 50 or 250 purifications. | description Reagents and consumables for organic solvent clean-up from DNA. 10, 50 or 250 purifications. | description Reagents and consumables for various tissue sample types. 96-well plate format. |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) - |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category | greener alternative category , Aligned | greener alternative category , Aligned |
| feature Typical/expected yield (Varies by sample. Please reference user guide for more information.), Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the removal of organic solvent traces from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Typical/expected yield (Varies by sample. Please reference user guide for more information.), Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purificiation of genomic DNA from human or animal tissue) |
| purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Time: 3 minutes or less), (Single-spin negative chromotography) | purified by (Single-spin negative chromotography) |
| storage temp. room temp | storage temp. room temp | storage temp. 2-8°C |
キットの構成要素のみ
製品番号
詳細
- DNA Cleanup Spin Columns
- 1x Tris Buffer
保管分類
10 - Combustible liquids
wgk
WGK 3
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Does the technology introduce any bias into the sample?
1 回答-
役に立ちましたか？
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 回答-
Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
役に立ちましたか？
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 回答-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
役に立ちましたか？
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 回答-
This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
役に立ちましたか？
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