E7407

ERK1、活性型、タグなし ヒト

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: MAPK3, P44ERK1, P44MAPK, PRKM3
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD03457429
• Specific activity: 329-445 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

特性

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 329-445 nmol/min·mg
• mol wt: ~44 kDa
• UniProt accession no.: P27361
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAPK3(5595)

詳細

General description

Extracellular signal-regulated kinase 1 (ERK1) belongs to mitogen-activated protein kinases (MAPK)/ERK family of intermediate proteins in the signaling pathways. This gene contains nine exons and eight introns, and spans 9kb. It is a constitutively expressed protein, the activity of which is altered by the phosphorylation state. It has a higher expression level in nervous tissues. It contains 11 kinase subdomains, common between humans and mice.[1] It has a molecular weight of 44kDa.[2]

Biochem/physiol Actions

ERK1は、多数の増殖因子やサイトカインに応答して活性化される細胞外シグナル制御キナーゼ(ERK)の一員であるタンパク質セリンスレオニンキナーゼです。ERK1の活性化には、チロシンとスレオニンの両方がMEKの媒介によってリン酸化される必要があります。ERK1は組織中に広範に分布しており、心臓、脳、脊髄で最も高度に発現しています。活性化されたERK1は核へ移行して様々な転写因子(Elk-1、c-Myc、c-Jun、c-Fos、C/EBPβなど)をリン酸化します。

ERK1 (MAPK3) participates in cellular signaling cascades that are activated in response to numerous growth factors and cytokines. Disruption of MAP kinase signaling functionality is associated with disease states including inflammation, cancer and neurodegenerative disease.

Physical form

25 mM MOPS溶液(pH 7.5、300 mM NaCl、0.25 mM DTT、0.1 mM EDTA、0.1 mM PMSF、25%グリセロール含有)。

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)