Yes, it would be recommended to use a different standard if available. Avian hemoglobin is quite different from mammalian hemoglobin, for which this reagent is primarily recommended. If the sample contains avian hemoglobin, it would be best to use a purified avian hemoglobin as the standard.
The human (Product H7379), bovine (Product H2500), or porcine (Product H4131) hemoglobin products recommended for use with this reagent are typically used for human, bovine, or porcine samples, respectively.
D5941
Drabkin試薬
for the determination of hemoglobin
別名:
ヘモグロビン定量試薬
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
6 vials | 在庫あり出荷可能from市川市大町368 | ￥48,900 |
この商品について
NACRES:
NA.25
UNSPSC Code:
12352402
Form:
liquid
Quality Segment
form
liquid
General description
Drabkin試薬は、血液試料中のヘモグロビン濃度の測定に使用します。これは、フェリシアン化カリウム、シアン化カリウムおよびリン酸二水素カリウムの成分からなります。フェリシアン化カリウムは、ヘモグロビンをメトヘモグロビンに、さらにシアンメトヘモグロビンに酸化します。波長530 nmでシアンメトヘモグロビン濃度を推定します。これは信頼性が高い方法であり、スルフヘモグロビン以外の全種類のヘモグロビンの測定が可能です。
Application
Drabkin試薬は、脳出血の評価のため脳組織で、[1]溶血の検出のため赤血球試料で、また対側および同側の脳組織ホモジネート試料で、それぞれ血中ヘモグロビンの比色定量に用いられています。
Preparation Note
炭酸水素ナトリウム、フェリシアン化カリウム、シアン化カリウムを含有します。
1 of 1
当該品目 | |||
|---|---|---|---|
| form liquid | form powder or crystals | form liquid | form - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
signalword
Danger
target_organs
Thyroid
supp_hazards
保管分類
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - STOT RE 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物
pdsc
第一種指定化学物質
prtr
名称等を表示すべき危険物及び有害物
ishl_indicated
名称等を通知すべき危険物及び有害物
ishl_notified
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Do I need a different hemoglobin standard for measuring hemoglobin concentration of avian blood?
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
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Should I first prepare a stock solution of human hemoglobin in water or PBS, or is it acceptable to dissolve human hemoglobin directly in Drabkin’s reagent, following the manufacturer’s recommendations to prepare a standard curve?
1 回答-
Hemoglobin should be dissolved in Drabkin's reagent as directed by the instructions in the technical bulletin. Please refer to the datasheet below for more details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdf役に立ちましたか？
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How does freezing affect the stability and shelf life of Drabkin's Solution? Would freezing help maintain its efficacy for a longer duration?
1 回答-
The stability of the Drabkin's solution when stored frozen has not been evaluated. The prepared solution is stable for at least 6 months at room temperature when stored protected from light. While it is possible that frozen storage would not impact the quality, this cannot be guaranteed. Please see the link below to review a publication that may be helpful.
'The effect of freezing on the potassium ferricyanide-potassium cyanide reagent used in the cyanmethemoglobin procedure for hemoglobin determination'
https://www.sciencedirect.com/science/article/abs/pii/0009898164901251役に立ちましたか？
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Can the concentration be calculated using Beer's law with the extinction coefficient of cyanmethemoglobin?
1 回答-
The Molar Extinction coefficient is necessary to determine the cyanmethemoglobin concentration according to the Beer Lambert's law. The extinction coefficient has been reported in several publications. Please see the link below to review an example:
https://academic.oup.com/ajcp/article-abstract/55/2/135/1769842Using the published coefficient at a specific wavelength, Beer's law may be used to calculate the concentration of cyanmethemoglobin in a given sample, based on its absorbance at that wavelength and the path length of the cuvette. The Drabkin's Reagent will allow for the determination of the total hemoglobin concentration from a linear calibration curve and the instructions for use for this product. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdf役に立ちましたか？
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Can the Drabkins reagent detect hemoglobin down to 0.05 mg/dL
1 回答-
The Drabkins reagent lacks the sensitivity to detect hemoglobin at levels as low as 0.05 mg/dl, with the approximate sensitivity being in the 3 to 4 mg/dl range.
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Is the hemoglobin (standard) provided suitable for use with Drabkin's Reagent (D5941)?
1 回答-
H2500 is recommended for use with Drabkin's reagent, as most mammalian hemoglobins are expected to yield similar absorbances with Drabkin reagent D5941, suggesting that porcine, rat, bovine, or human hemoglobin standards may also work. Additionally, a specific Porcine hemoglobin product, H4131, is available.
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How to store the configured drabkin reagent and how long it can be stored
1 回答-
Store the Drabkin’s Reagent at room temperature protected from light. The powdered Drabkin’s Reagent is stable for at least 2 years. The prepared Drabkin’s Solution is stable for at least 6 months when stored at room temperature and protected from light (amber bottle).
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what is the dilution ration that you are using for drabkin's reagent to distilled water? 1:40? Since you can't tell me what the volume is for one vial of the reagent
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A vial of Drabkin's reagent D5941 contains 1.25 g of the powder mixture as follows:
Sodium bicarbonate, CAS # 144-55-8 || 80% (w / w) or 1000 mg per vial
Potassium ferricyanide, CAS # 13746-66-2 || 16% (w / w) or 200 mg per vial
Potassium cyanide, CAS # 151-50-8 || 4% (w / w) or 50 mg per vialThe vial should be reconstituted with 1,000 mL of water and 0.5 mL of 30 % Brij L23 Solution.
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