CS0004

ミクロコッカスヌクレアーゼ Staphylococcus aureus由来

With reaction buffer and BSA solution

• 別名: MNase, エンドヌクレアーゼ, ミクロコッカス由来, ミクロコッカスエンドヌクレアーゼ
• CAS番号: 9013-53-0
• UNSPSC Code: 12352202
• NACRES: NA.77
• EC Number: 3.1.31.1 (BRENDA, IUBMB)
• MDL number: MFCD00131731
• Biological source: Staphylococcus aureus

特性

• biological source: Staphylococcus aureus
• form: liquid
• input: bacteria and yeast sample(s)
• shipped in: wet ice
• storage temp.: −20°C

詳細

General description

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Application

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Biochem/physiol Actions

DNAの5′-ホスホジエステル結合を加水分解します。

Other Notes

注:1 μmolユニット = 約85 A₂₆₀ユニットです。ここで、1 A₂₆₀ユニットは、反応液量が3.55 mLの場合に、pH 8.8、37°C、30分間に、ΔA₂₆₀が1.0となる酵素量です。光路長 = 1 cm。

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3