PEA-10

97040310, mouse unknown/unspecified, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: PEA-10, 97040310
• 由来生物: mouse unknown/unspecified
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse embryo fibroblast, LRP-deficient, heterozygous

細胞株の説明

The cell line PEA-10 is heterozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-10 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Together with the cell lines PEA-13 (Sigma Catalogue number. 97040311) and MEF-1 (Sigma Catalogue number. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP deficiency. This is of importance since LRP deficient mouse embryos die early during gestation.

アプリケーション

Study of LRP and LRP deficiency.

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split confleunt cultures 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

その他情報

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