ATHOS

93122325, Porcine thyroid, Epithelial

• UNSPSCコード: 41106514

特性

• product name: ATHOS, 93122325
• 由来生物: Porcine thyroid
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Thyroglobulin, plasminogen activator (u-PA)
• 受容体: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Porcine thyroid epithelial

細胞株の説明

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

アプリケーション

Mechanism of thyroid follicle reconstruction

培地

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

その他情報

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