PANC-1 Cell Line human

87092802, human pancreas, Epithelial

• 別名: PANC1 Cells, Panc-1-P Cells
• UNSPSCコード: 41106514
• eCl@ss: 32190102

特性

• product name: PANC-1 Cell Line human, 87092802, from human pancreas
• 由来生物: human pancreas
• 成長モード: Adherent
• 核型: 2n = 46, hypertriploid
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian pancreas

細胞株の説明

Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

アプリケーション

Human PANC-1 cell line has been used to study the role of the intermediate filament keratin reorganization on the alignment and migration of cancer cells. It has also been used to study calcium-mediated actin reset (CaAR) in response to physiological changes.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8,10
THO1: 7,8
TPOX: 8,11
vWA: 15

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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