KHOS-240S

86112810, human bone, Epithelial

• UNSPSCコード: 41106514

特性

• product name: KHOS-240S, 86112810
• 由来生物: human bone
• 成長モード: Adherent
• 核型: 2n = 46, aneuploid
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian osteosarcoma

細胞株の説明

A revertant line of the KHOS/NP cell line (Sigma Catalogue number. 84102903). Established after overnight incubation at 40.5°C and then sub-cloned at 36°C. The line has similar properties to the parent HOS cell line. Cells are non-tumourigenic in nude mice and no longer possess a rescuable MSV genome.

アプリケーション

Tumourigenicity, transformation, biochemical and virus studies: MSV

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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