Detroit 551

86082802, human skin (embryonic), Fibroblast

• UNSPSCコード: 41106514

特性

• product name: Detroit 551, 86082802
• 由来生物: human skin (embryonic)
• 成長モード: Adherent
• 核型: 2n = 46
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian embryo skin

細胞株の説明

Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.

アプリケーション

Chromosome and virus studies (Normal control): herpesviruses, SV40

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; at most 25 subcultures from original tissue.

その他情報

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