W3/25

Reactivity: Anti-CD4, rat, 84112002

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髄腫: NS1/1
• 反応性: Anti-CD4, rat
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse NS1/1 x Mouse BALB/c spleen

細胞株の説明

The antibody is directed against surface antigens found on 86% of rat thymocytes, 70% of peripheral T lymphocytes and macrophages. The antigen has a molecular weight of approximately 50kDa. W3/25 positive T cells have been identified as T helper cells.

免疫原

Rat thymocyte membrane

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

その他情報

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免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.