Ma-Mel-19

13012460, human skin, Epithelial

• UNSPSCコード: 41106514

特性

• product name: Ma-Mel-19, from human skin, 13012460
• 由来生物: human skin
• 詳細: Human melanoma, metastasis
• 成長モード: Adherent
• 形態: Epithelial
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の説明

Ma-Mel-19 (ESTDAB-152) was established from the cutaneous/subcutaneous metastasis in a 62 year-old female patient with metastatic melanoma (superficial spreading melanoma stage IV). The Ma-Mel-19 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission′s fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

DNAプロファイル

STR-PCR Data:

Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11,13
D5S818: 9,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 14,18

培地

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 8 x10,000 cells/cm² .

その他情報

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.