1.1E7

10070101, human pancreas (islets), Epitheloid

• UNSPSCコード: 41106514

特性

• product name: 1.1E7, from human pancreas(islets), 10070101
• 由来生物: human pancreas (islets)
• 成長モード: Adherent
• 核型: Modal chromosome number 67-71
• 形態: Epitheloid
• 製品: 1.1E7 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice

詳細

細胞株の説明

1.1E7 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802). 1.1E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.1E7 cells provide a method of producing pure insulin secreting cells when stimulated . This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNAプロファイル

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8
THO1: 7,8
TPOX: 8,11
vWA: 15

培地

RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:6 , i.e., seeding at 2-4 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 23hrs. At confluence 10⁵ cells/cm² can be expected.

その他情報

HPA Culture Collections由来でSIGMAによって供給される培養物は研究の目的のみに使用できます。商業目的での細胞株の使用に関する質問は、その細胞株のデポジターに照会されます。一部の細胞株には、細胞株の供給の前に受給予定者によって物質移動契約書への記入を完了させるという要求など、さらなる特別な公開条件があります。詳しくはTerms & Conditions of Supplyをご覧ください。

法的情報

Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).