108CC15

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Rat Hybridoma nerve
• 包装: tube of 5 μg 08062516-DNA-5UG; pkg of vial of cells 08062516-1VL
• 成長モード: Adherent
• 核型: Modal No. varied with passage see reference
• 形態: Neuronal
• 製品: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• 受容体: Receptors for neurohormones: delta opioid, PGE1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse neuroblastoma x Rat glioma hybrid

細胞株の説明

The cell line108CC15 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-15 by Klee and Nirenberg. Sigma has two catalogue entries for the 108CC15 cell line which differ in that they were deposited by different groups: 108CC15 from the original group that derived the cell line (Sigma Catalogue number 08062516) and NG108-15 (Sigma Catalogue number 88112302). Several other cell lines in the Sigma catalogue have been derived from 108CC15. This is a cholinergic hybrid cell line.

アプリケーション

Used as a neuronal Model

培地

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

その他情報

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