KARPAS-25

6072601, human lung (effusion), Round to polygonal cells growing singly in suspension

• 別名: KARPAS 25, KARPAS, 25, KARPAS25
• UNSPSCコード: 41106514

特性

• product name: KARPAS-25, 06072601
• 由来生物: human lung (effusion)
• 成長モード: Suspension
• 核型: Hyperdiploid with complex aberrant chromosomes
• 形態: Round to polygonal cells growing singly in suspension
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice

詳細

細胞株の由来

Human acute plasma cell leukaemia

細胞株の説明

Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.

培地

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

継代と培養方法

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

その他情報

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免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.