22Rv1

5092802, human prostate, Epithelial

• 別名: 22Rv-1 Cells, CWR-22rv1 Cells, CWR22-R1 Cells
• UNSPSCコード: 41106514

特性

• product name: 22Rv1, 05092802
• 由来生物: human prostate
• 成長モード: Adherent aggregates
• 核型: 49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]
• 形態: Epithelial
• 製品: Not specified
• 受容体: androgen receptor positive
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Xenograft Prostate

細胞株の説明

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor β-1 (TGF β-1). This cell line is tumorigenic in nude mice.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21

培地

RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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