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由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
DCS-270, monoclonal
形状
buffered aqueous solution
分子量
antigen 61 kDa
化学種の反応性
human
濃度
~1 mg/mL
テクニック
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using using whole extract of cultured 293T (human embryonal kidney) cells
アイソタイプ
IgG2a
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CHEK2(11200)
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詳細
The protein encoded by this gene is a serine/threonine-protein kinase that acts as a cell cycle checkpoint regulator and a putative tumor suppressor. It contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA), which is activated by rapid phosphorylation in response to replication blocks and DNA damage.
特異性
本抗体に認識されるエピト-プは、Chk2分子のN末端調節ドメイン内に存在します。
免疫原
リコンビナントヒトChk2。
アプリケーション
Monoclonal Anti-Chk2 antibody produced in mouse is suitable for immunocytochemistry, immunohistochemistry (formalin-fixed, paraffin-embedded sections), microarray, immunoprecipitation and for immunoblotting at a concentration of 1-2 μg/mL using whole extract of cultured 293T cells. It was used for Chk2 immunoprecipitation experiments to study the engagement of the ATR-dependent DNA damage response at the human papillomavirus 18 replication centers during the initial amplification
生物化学的／生理学的作用
The protein propagates signals from damaged or unreplicated DNA. It is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.
物理的形状
0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C9233-BULK:
C9233-.2ML:
C9233-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
C H Lee et al.
The Journal of biological chemistry, 276(32), 30537-30541 (2001-06-08)
hCds1 (Chk2) is an evolutionarily conserved kinase that functions in DNA damage response and cell cycle checkpoint. The Cds1 family of kinases are activated by a family of large phosphatidylinositol 3-kinase-like kinases. In humans, ataxia telangiectasia-mutated (ATM) and ataxia-telangiectasia and
Yin Liu et al.
Human mutation, 32(9), 1000-1003 (2011-05-28)
The association between the CHEK2 and breast cancer risk in Chinese women is unknown. Here, we screened the full CHEK2 coding sequence in 118 Chinese familial breast cancer cases who are negative for mutations in BRCA1 and BRCA2, one recurrent
Tormi Reinson et al.
Journal of virology, 87(2), 951-964 (2012-11-09)
We have previously demonstrated that the human papillomavirus (HPV) genome replicates effectively in U2OS cells after transfection using electroporation. The transient extrachromosomal replication, stable maintenance, and late amplification of the viral genome could be studied for high- and low-risk mucosal
A L Brown et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(7), 3745-3750 (1999-03-31)
Checkpoints maintain the order and fidelity of the eukaryotic cell cycle, and defects in checkpoints contribute to genetic instability and cancer. Much of our current understanding of checkpoints comes from genetic studies conducted in yeast. In the fission yeast Schizosaccharomyces
Vidar Staalesen et al.
Oncogene, 23(52), 8535-8544 (2004-09-14)
The DNA damage checkpoint kinase, CHK2, promotes growth arrest or apoptosis through phosphorylating targets such as Cdc25A, Cdc25C, BRCA1, and p53. Both germline and somatic loss-of-function CHEK2 mutations occur in human tumours, the former linked to the Li-Fraumeni syndrome, and
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