C4290

ブチリルコリンエステラーゼ ウマ血清由来

lyophilized powder, ≥500 units/mg protein

• 別名: アシルコリンアシルヒドロラーゼ, コリンエステラーゼ, ブチリル, シュードコリンエステラーゼ
• CAS番号: 9001-08-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-579-2
• MDL number: MFCD00081455
• EC Number: 3.1.1.8 (BRENDA, IUBMB)
• Specific activity: ≥500 units/mg protein
• Biological source: equine serum

特性

• biological source: equine serum
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• composition: Protein, ≥10%
• storage temp.: −20°C

詳細

Application

Butyrylcholinesterase (BChE) from equine serum has been used:

• to determine the inhibitory concentration of bupivacaine on butyrylcholinesterase
• in acetylcholinesterase (AChE)/BChE activity assay to determine the inhibitory activity of benzothiazole-piperazine compounds[1]

Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity shows promise. There is a correlation between the level of BChE in human blood and degree of protection against potentially toxic nerve agents.[2] There has also been interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals.[3] The enzyme has been used to test bupivacaine as an inhibitor of butyrylcholinesterase during acetylcholinesterase assay using cerebrospinal fluid.[4]

Biochem/physiol Actions

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca²⁺ and Mg²⁺ and the activity is constant over the pH range 6.0-8.0. It is inhibited by Betaine, nicotine, organophosphates, carbamates.

Physical form

バッファ-塩を含有する高純度の凍結乾燥品

Analysis Note

タンパク量はビウレット法で測定

Other Notes

1ユニットは、pH 8.0、37°C、1分間に、1.0 μmolのブチリルコリンをコリンと酪酸とに加水分解する酵素量です。ブチリルコリンを基質とした場合の活性は、アセチルコリンを基質とした場合の約2.5倍となります。

安全性情報

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• 保管分類: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)