C2409
クレアチナーゼ from Actinobacillus sp.
lyophilized powder, ≥4 units/mg solid
別名:
クレアチンアミジノヒドロラーゼ
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1000 units | カートの在庫状況を確認する | ￥81,300 |
この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-471-1
MDL number:
Specific activity:
≥4 units/mg solid
Biological source:
bacterial (Actinobacillus spp.)
￥81,300
カートの在庫状況を確認する
biological source
bacterial (Actinobacillus spp.)
Quality Segment
form
lyophilized powder
specific activity
≥4 units/mg solid
mol wt
~100 kDa
storage temp.
−20°C
General description
Creatinase is a homodimer that catalyzes hydrolysis of creatine.[1] It consists of two monomer subunits and two defined domains; N and C terminal domains.[2] The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.[3]In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent.[4]
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾2M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu++, Hg++, Ag+
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)
Michaelis constant: 1.9 x 10‾2M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu++, Hg++, Ag+
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)
Application
Biochem/physiol Actions
Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.[7]
Physical form
グルコン酸、リン酸カリウム、EDTAを含有する凍結乾燥粉末です。
Other Notes
1ユニットは、pH 7.5、37°C、1分間で、1.0 μmolのクレアチンを尿素とサルコシンに加水分解する酵素量です。
1 of 1
当該品目 | |||
|---|---|---|---|
| specific activity ≥4 units/mg solid | specific activity 100-300 units/mg protein | specific activity ≥50 units/mg protein | specific activity ≥300 U/mg, 25 °C, 750 U/mg, 37 °C |
| biological source bacterial (Actinobacillus spp.) | biological source - | biological source Escherichia coli | biological source rabbit skeletal muscle |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
| mol wt ~100 kDa | mol wt ~175 kDa | mol wt 110 kDa | mol wt - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)