B3679
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
別名:
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
100 mL | カートの在庫状況を確認する | ￥37,700 |
この商品について
UNSPSC Code:
12352204
NACRES:
NA.83
￥37,700
カートの在庫状況を確認する
製品名
BCIP®/NBT-Purple 膜用液体基質システム, ready to use alkaline phosphatase substrate
Quality Segment
form
liquid
storage temp.
2-8°C
Application
BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Physical form
そのままお使いいただけます。
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
2～8°Cで保存すると1年間は安定です。
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当該品目 | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 300 | Quality Level 100 | Quality Level - |
| form liquid | form liquid | form liquid | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable