B0555

モノクロナール抗血管内皮細胞増殖因子受容体2 マウス宿主抗体

clone 260.4, KDR-2, purified from hybridoma cell culture

• 別名: 抗KDR抗体, 抗VEGFR-2抗体
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: biotin conjugate
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 260.4, KDR-2, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human
• テクニック: western blot: 1:1,000 using insect cell culture supernatant expressing human extracellular recombinant VEGF R
• アイソタイプ: IgG1
• UniProtアクセッション番号: P35968
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... KDR(3791)

詳細

詳細

Biotin Conjugated Monoclonal Anti-VEGF Receptor-2 (KDR) (mouse IgG1 isotype) is derived from the KDR-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified recombinant human extracellular VEGF receptor-2 (KDR). Vascular endothelial growth factor (VEGF) is also called vasculotropin (VAS), vascular permeability factor (VPF) and kinase-insert domain containing receptor (KDR8). It is a member of a family of endothelial cell mitogens and angiogenic factors. VEGF is a homodimeric heparin-binding glycoprotein. The KDR gene has been mapped to human chromosome 4q11-q12. The homologous gene for the human KDR gene is mouse Flk1 (fetal liver kinase 1) and rat TKrc.

特異性

ヒトVEGFレセプタ-2と特異的に反応します。こののクロ-ン番号は260.4とも表記されます。Flt1(VEGFR-1)、Flt4(VEGFR-3)、血小板由来増殖因子レセプタ-(PDGF-R)は認識しません。

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody is purified from ascites fluid, and conjugated withSulfo-NHS-LC-Biotin. It is specific for human VEGF receptor2. It does not cross react with Flt1, Flt4 or platelet-derived growth factor receptor β (PDGF-R1).

免疫原

精製リコンビナントヒト細胞外VEGFレセプタ-2(KDR)。

アプリケーション

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody produced in mouse has also been used in :

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• flow cytometry
• immunohistochemistry
• immunohistochemistry

生物化学的／生理学的作用

Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF that plays important role in embryonic vasculogenesis, angiogenesis and homeostasis. VEGF R2 has the ability to form heterodimers with VEGF R1 and VEGF R3. The binding of VEGF to VEGF R2 results in activation of tyrosine amino acids of the cytoplasmic tail of the receptor. This interaction results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R2 include in epithelial cell growth, proliferation, survival, migration and vascular permeability and angiogenesis. Due to its role in promoting migration and angiogenesis, VEGF R2 has been implicated in breast and prostate cancer.

ターゲットの説明

KDRレセプタ-(VEGFRa)は造血幹細胞(HSC)のマ-カ-とされており、多能性のHSCと、系譜が約束されたHSC前駆体とは区別されます。

物理的形状

PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable