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Merck

53654

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 600 antibody produced in goat

for STED application

別名:

Abberior® STAR 600-Anti-Rabbit IgG antibody produced in goat

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About This Item

UNSPSCコード:
12352202
NACRES:
NA.45

由来生物

goat

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

secondary antibodies

クローン

polyclonal

形状

buffered aqueous solution

化学種の反応性

rabbit

利用可能性

available only in USA and Canada

濃度

~1 mg/mL

蛍光検出

λex 604 nm; λem 623 nm in PBS, pH 7.4

保管温度

−20°C

詳細

Abberior STAR 600 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 600 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be effectively excited with excitation light between 550 - 610 nm. Abberior STAR 600 can substitute dyes like Atto 590, Atto 594 or Alexa Fluor 594. For STED microscopy, Abberior STAR 600 can be most efficiently used with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 600 performs exceptionally well in combination with Abberior STAR 635P, STAR 635 or STAR RED. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 604 (PBS pH 7.4), 604 (H2O), 601 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: : 95 000 (PBS pH 7.4), 95 000 (H2O), 100 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,25 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 623 (PBS pH 7.4), 623 (H2O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)

適合性

Designed and tested for fluorescent super-resolution microscopy

アナリシスノート

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

法的情報

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Tobias Müller et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(8), 1986-2000 (2012-03-01)
For about a decade, superresolution fluorescence microscopy has been advancing steadily, maturing from the proof-of-principle stage to routine application. Of the various techniques, STED (stimulated emission depletion) microscopy was the first to break the diffraction barrier. Today, it is a

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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