The dye:protein ratio for live cell imaging has not been confirmed. According to literature references, NTA-Atto 488 for live imaging, used for immunofluorescence or staining His-tagged proteins on cells, typically involves dilutions of 1:200 to 1:1000 of the reconstituted dye (1 mg/mL) in buffer.
39625
NTA - Atto 488
BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)
別名:
Atto 488 - ニトリロ三酢酸、Ni2+ - イオン錯体, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
250 μg | 在庫あり出荷可能from市川市大町368 | ￥45,700 |
この商品について
NACRES:
NA.32
UNSPSC Code:
12171501
product line
BioReagent
Quality Segment
assay
≥80% (sum of isomers, HPCE)
form
solid
fluorescence
λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
General description
詳しくは こちらをご覧ください。
Application
HISタグ付きタンパク質の蛍光検出用
Legal Information
本製品は研究専用です。商業目的で使用する場合は、ライセンス取得について知的所有権（IP）保持者（ATTO-TEC GmbH、ドイツ）にお問い合わせください。
1 of 1
当該品目 | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide | fluorescence λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide | fluorescence λex 647 nm; λem 669 nm in PBS | fluorescence - |
| form solid | form lumps | form - | form powder |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| assay ≥80% (sum of isomers, HPCE) | assay ≥80% (sum of isomers, HPCE) | assay ≥80% (HPCE) | assay ≥90% (HPLC), ≥90% (degree of coupling) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
保管分類
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
特定第一種指定化学物質
prtr
名称等を表示すべき危険物及び有害物
ishl_indicated
名称等を通知すべき危険物及び有害物
ishl_notified
39625-250UG-F:4548173296135 + 39625-BULK-F: + 39625-250UG-BULK-F:
jan
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What is the dye:protein ratio used for live cell imaging?
1 回答-
役に立ちましたか？
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Can this reagent detect Histidine affinity tag (HAT)? What is the dilution ratio for WB or IF?
1 回答-
This product is provided as 250 micrograms of powder per vial. The contents of the vial can be reconstituted in 250 microliters of phosphate buffered saline (PBS) to make a 1 mg/mL stock solution. Dilutions of 1:1000 to 1:2500 in PBS with TWEEN 20 (PBS-T; Product P3563 at 1X) can be used to stain histidine-tagged proteins by Western Blot (WB).
Immunofluorescence (IF) is typically less sensitive than WB, so dilutions of 1:200 to 1:1000 may be appropriate.
See: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-labeling-and-modification/ni-nta-atto-conjugates役に立ちましたか？
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Is this product suitable for detection of polyhistidine tagged proteins directly in SDS-PAGE or Western blot?
1 回答-
ATTO 488 NHS-ester can be used for the fluorescence detection of HIS-tagged proteins and polyhistidine tagged proteins directly in SDS-PAGE or Western blot.
役に立ちましたか？
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We have a his-tag labeled recombinant protein that we would like to have fluorescently labeled, before we use it to detect/label antibodies. I came across Ni-NTA-Atto Conjugates and I was wondering wether we could use this to label out his-tagprotein?
1 回答-
There should be no difficulty with the use of the product to label a HIS-tagged recombinant protein as long as the HIS-6 tag is in an acceptable position for binding. Typically, this is done with either a N or a C-terminal His6 tag. Please note that the only tests that we conduct on this material include a solubility, purity and a luminescence assay. Hence, we do not offer a detailed protocol for use of the product for HIS-tag protein labeling.
役に立ちましたか？
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What is the number of nickel ions present in product 39624? Is it mono-nta, tris-nta, or another form?
1 回答-
There is one nickel ion per molecule, but the exact structure is proprietary.
役に立ちましたか？
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What is the molecular weight of this product NTA-ATTO488?
1 回答-
The molecular weight of this product is 964 Da.
役に立ちましたか？
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