304M-1

Nerve Growth Factor Receptor (NGFR) (MRQ-21) Mouse Monoclonal Antibody

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100; 500
• 結合体: unconjugated
• 抗体製品の状態: diluted ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: MRQ-21, monoclonal
• 詳細: For In Vitro Diagnostic Use in Select Regions (See Chart)
• 形状: buffered aqueous solution
• 化学種の反応性: human
• 包装: vial of 0.1 mL concentrate (304M-14); vial of 0.5 mL concentrate (304M-15); bottle of 1.0 mL predilute (304M-17); vial of 1.0 mL concentrate (304M-16); bottle of 7.0 mL predilute (304M-18)
• メーカー／製品名: Cell Marque^™
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• アイソタイプ: IgG1
• コントロール: breast
• 輸送温度: wet ice
• 保管温度: 2-8°C
• 視覚化: cytoplasmic
• 遺伝子情報: human ... NGFR(4804)

詳細

詳細

NGFR, a 75 kd glycoprotein (also known as P-75NTR), is the first of neurotrophin receptors to be isolated and is a member of the tumor necrosis factor (TNF) receptor family. It is expressed not only in sympathetic and sensory neurons, but also in various neural crest cell or tumor derivatives such as melanocytes, melanomas, neuroblastomas, pheochromocytomas, neurofibromas, and neurotized nevi (type C melanocytes). NGFR has been shown to be a reliable marker for desmoplastic and neurotropic melanoma by several groups. Although the sensitivity of NGFR staining in desmoplastic melanomas has not been systematically analyzed, all reported cases stain with NGFR. A review of 9 cases of desmoplastic melanomas diagnosed in the Dermatopathology Section of the Department of Dermatology at Boston University School of Medicine between 2001 and 2004 disclosed that all 9 cases of desmoplastic melanomas tested stained positively with NGFR. These findings are in contrast to a study reported by Huttenbach et al in which only 33% of their desmoplastic melanomas stained with NGFR. This difference emphasizes the importance of the choice of monoclonal antibody to obtain consistent results. This staining property of desmoplastic melanoma cells can be useful in diagnosing challenging cases, namely early lesions, suspected cases with negative or weak S-100 staining, and those that must be distinguished from a scar. It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature nonneural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells. Unlike the high-affinity nerve growth factor tyrosine kinase receptors (TrkA, TrkB and TrkC), NGFR has no intrinsic tyrosine kinase activity. Studies in prostate and urothelial cancer suggest that NGFR may act as a tumour suppressor, negatively regulating cell growth and proliferation. Anti-NGFR labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts and thus aides in the diagnosis of malignancy in the breast. Associated products: GFAP, S.M. Myosin, Calponin, NSE, Myelin Basic Protein, S-100, HMB45, Tyrosinase, MART-1

品質

IVD

IVD

IVD

RUO

関連事項

Nerve Growth Factor Receptor (NGFR) Positive Control Slides, Product No. 304S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

物理的形状

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

調製ノート

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

その他情報

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

法的情報

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany