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product name
E11, from Snakehead fish
由来生物
fish fish (whole)
品質水準
成長モード
Adherent
核型
Not specified
形態
Fibroblast
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Snakehead fish, whole fry tissue
細胞株の説明
E11 is a clone of the cell line SSN-1 and is persistantly infected with a C-type retrovirus (SnRV). It is susceptible to piscine nodavirus strains belonging to different genotypes (SJNNV, RGNNV, TPNNV and BFNNV - striped jack, red spotted grouper, tiger puffer and barfin flounder nervous necosis viruses repectively). E11 is highly permissive to nodavirus infection and production. An advantage of E11, over the parental line SSN-1, is its steady, faster growth to confluency and longer stability in monolayer cultures for 2 weeks at 25 °C or 4 weeks at 20 °C and its reproducible, clear CPE characterised by cytoplasmic vacuole formation followed by intensive disintegration.
培地
L15 + 5% Fetal Bovine Serum (FBS)+ 2 mM Glutamine
継代と培養方法
Split sub-confluent cultures (70-80%) 1:4, ie., seeding at 3 x10,000 cells/cm2 using 0.05% trypsin/EDTA. No CO2. Culture at 25 °C
その他情報
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Professor Toshihiro Nakai, PhD Laboratory of Fish Pathology Graduate School of Biosphere Science Hiroshima University 1-4-4 Kagamiyama, Higashi-Hiroshima Hiroshima 739-8528, Japan
For complete product information, please see ECACC
免責事項
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
01110916-1VL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Pitchaporn Waiyamitra et al.
Pathogens (Basel, Switzerland), 9(11) (2020-11-12)
Tilapia lake virus (TiLV) causes an emerging viral disease associated with high mortality and economic damage in tilapia farming around the world. The use of probiotics in aquaculture has been suggested as an alternative to antibiotics and drugs to reduce
T Iwamoto et al.
Diseases of aquatic organisms, 43(2), 81-89 (2001-01-06)
Six cell clones were derived from the SSN-1 cell line, which is composed of a mixed cell population and persistently infected with a C-type retrovirus (SnRV). These clones were susceptible to 4 piscine nodavirus strains belonging to different genotypes (SJNNV
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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